Food safety is a top priority for the food industry, and the rapid and reliable detection of pathogens in food products and their environment is essential for ensuring the quality and safety of goods. At Loop Dee Science, we have always strived to offer innovative solutions that support food manufacturers in their processes of quality control and microbial risk prevention. It is with this goal in mind that we are proud to announce the launch of our new detection solution for Listeria spp., a critical target for environmental monitoring.

In this article, we will provide a detailed overview of this new target, its benefits and functionality, and why it represents a major advancement for the food industry.

Why is it important to detect Listeria spp. in environmental controls?

Listeria monocytogenes is a well-known pathogenic bacterium responsible for listeriosis, a potentially serious foodborne infection, particularly dangerous for vulnerable populations (pregnant women, the elderly, and immunocompromised individuals). However, Listeria spp., which includes several species closely related to Listeria monocytogenes, is also a concern in the food sector. These bacteria can thrive in a variety of environments, particularly in processing equipment, work surfaces, and storage areas, where they can act as a source of cross-contamination.

The detection of Listeria spp. in environmental controls is, therefore, of paramount importance for food manufacturers. By quickly identifying the presence of these bacteria, it is possible to prevent further contamination of food products, maintain a safe production environment, and comply with the strict food safety regulations in place.

Traditional methods for detecting Listeria spp. require lengthy enrichment steps followed by confirmation tests, often taking more than 48 hours to produce results. In a food production environment, this delay is too long and can pose significant risks. That’s why a rapid and precise method for detecting Listeria spp. in self-monitoring is crucial for the proactive management of food safety.

A kit compliant with the protocols of other targets

Our new Listeria spp. detection kit follows the same proven protocols as those already used for our other targets, such as Salmonella spp., E. coli, Enterococcus faecalis, and Listeria monocytogenes. This approach ensures ease of use and standardization across our entire range of solutions.

The protocol for our kit is designed to be easily integrated into the control practices of food manufacturers, particularly for samples following enrichment. Whether for raw materials, semi-finished products, or finished products, our Listeria spp. detection kit works intuitively and quickly, while ensuring optimal performance.

How the kit works and integration with our LoopiX device

One of the key strengths of our solution is its integration with the LoopiX , a device that automates the detection process to facilitate analysis and minimize the risk of human error. The Listeria spp. detection kit, like all the other targets in the range, is designed to work seamlessly with the LoopiX device.

The LoopiX performs thermal lysis extraction, target-specific amplification, reading, and interpretation of results. This system is designed to be user-friendly, even for those with no laboratory or molecular biology experience. The user prepares the sample in a simple and quick manner (in under 10 minutes), and only basic training is required to carry out the analyses.

Additionally, LoopiX can be controlled via a smartphone application, the LoopiX App, and an online software platform, LoopiX Cloud. These tools allow users to configure the LoopiX system for analysis of all targets in the range (including Listeria monocytogenes, Salmonella spp., etc.). They also enable the storage and centralization of test results for traceability purposes.

Validation by an accredited external laboratory: perfect alignment

To ensure the performance and reliability of our new detection kit, we subjected the product to comparative testing against the reference method, performed according to ISO standards. These tests were conducted by an accredited external laboratory, specializing in microbiological testing for the food industry.

The results of these comparisons were flawless: our detection kit demonstrated a 100% sensitivity and specificity, meaning it can detect all occurrences of Listeria spp. without generating false positives. This makes our solution an extremely reliable detection method, perfectly suited to the safety and compliance requirements of the food industry.

This external laboratory validation further guarantees the robustness of our solution and its alignment with industry standards. Our clients can, therefore, have complete confidence in the performance of our kit for quickly and efficiently detecting Listeria spp.

Key benefits of our Listeria spp. detection kit

  1. Rapid and efficient: Results in 1h30 with LoopiX, compared to a minimum of 24 hours with traditional methods.
  2. 100% sensitivity and specificity: Reliable detection without false positives or false negatives, validated by an accredited external laboratory.
  3. Ease of use: Same as our other detection kits, allowing easy integration into routine control practices.
  4. Versatility: Suitable for detecting Listeria spp. in enriched samples (raw materials, semi-finished products, and finished products), as well as for environmental monitoring, including surface swabbing.
  5. Risk reduction: By quickly detecting Listeria spp., you can take proactive steps to prevent contamination of your food products, ensuring the safety of your consumers.
  6. Compliance with standards: Our method complies with the relevant industry regulations, and external validation of its performance guarantees its effectiveness in a regulated environment.

Conclusion: a major development for the industry

With the launch of our Listeria spp. detection kit, we further strengthen our commitment to providing rapid, reliable solutions tailored to the specific needs of the food industry. This new kit, combined with our LoopiX device, represents a true breakthrough in environmental control practices.

Food manufacturers no longer have to choose between speed and accuracy: they can now have both. With this solution, it becomes possible to prevent the risks of contamination by Listeria spp., ensure a production environment that complies with food safety standards, and respond effectively to the challenges of the modern food industry.

We are proud to support our clients in achieving safer, faster, and more efficient production with this new solution.